Investigation of the optimized dextran-degrading enzyme conditions on the decomposition of different molecular weights of pure dextran using response surface methodology

The elimination of various molecular weights of contaminated dextran in sugar processing is quite complicated, especially using enzymatic decomposition. Many factors including enzyme concentration, retention time, pH, temperature, and sucrose concentration affect enzyme hydrolysis. 

The optimizing conditions of various molecular weights of dextran removal were investigated using response surface methodology. The optimum hydrolyzed conditions of 1,500? G/mL of all molecular weights of pure dextran from the one-factor-at-a-time (OFAT) experiments were: dextranase (Amano Enzyme Inc., Japan) at 5-15 ppmtotal soluble solids for 5-15 min at 50-65? C with pH 4.5-6.5 and sucroseconcentration at 15-25%. The results from the OFAT experiments used a Box-Benhken experimental design with four factors and three levels that achieved fit models that predicted the dextran removal under different conditions of various molecular weights of pure dextran.