Molecular Identification of Rotylenchulus Reniformis (Nematoda) By Using Its Region of Rdna

Reniform nematode (Rotylenchulus reniformis) is widely distributed in all cotton (Gossypium spp.) grown regions of India. In Reniform nemotode infection, the growth of cotton roots and shoots was reduced at level of more than 8 and 1 nematode per g soil, respectively. DNA-based Molecular identi?cation of Plant parasitic nematode species has been determined by Internal Transcribed spacer (ITS) gene with PCR Techniques. 

The ribosomal DNA (rDNA) is a novel genetic marker and it is a multi-gene family consisting of many copies (100-500 in animals) of genes encoding for three ribosomal components 28S, 5.8S and 18S. Phylogeny is the history of organism lineage as they change through time. It implies that different species arise from previous forms via decent and that all organisms from the smallest microbes to the largest plants and vertebrates were connected by the passage by genes along the branches of the phylogenetic tree that links all the life form.